A luminescent G-quadruplex switch-on probe for the highly selective and tunable detection of cysteine and glutathione.
نویسندگان
چکیده
A G-quadruplex-selective luminescent iridium(III) switch-on probe has been developed for the detection of cysteine (Cys) in aqueous solution. The system is highly sensitive and selective towards Cys with a tunable range of detection. The detection of glutathione (GSH) is also examined.
منابع مشابه
A highly selective G-quadruplex-based luminescent switch-on probe for the detection of gene deletion.
A G-quadruplex-based switch-on luminescence assay has been developed for the detection of gene deletion using a cyclometallated iridium(III) complex as a G-quadruplex-selective probe. Upon hybridization with the target DNA, the two split G-quadruplex-forming sequences assemble into a split G-quadruplex, which greatly enhances the luminescence emission of the iridium(III) probe. The assay is sim...
متن کاملA selective G-quadruplex-based luminescent switch-on probe for the detection of nanomolar silver(I) ions in aqueous solution.
A G-quadruplex-based luminescent platinum(II) switch-on probe has been developed for the selective detection of nanomolar Ag(+) ions in aqueous solution.
متن کاملAn oligonucleotide-based label-free luminescent switch-on probe for RNA detection utilizing a G-quadruplex-selective iridium(III) complex.
We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(iii) complex for the construction of an oligonucleotide-based, label-free, rapid and convenient luminescent RNA detection platform.
متن کاملLabel-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe.
A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.
متن کاملDetection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe.
We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 49 8 شماره
صفحات -
تاریخ انتشار 2013